Enzymes and Inhibitors
Apexbio Technology LLC Phosphatase Inhibitor Cocktail 2 (100X in ddH2O) 100x1ml
Many cellular processes are regulated by reversible phosphorylation of proteins and approximately 30% of proteins may become phosphorylated during their lifetime Cellular crude extracts contain numerous endogenous enzymes such as phosphatases and proteases which can degrade and modify proteins present in the extract Addition of inhibitors for these enzymes is the optimal approach for maximizing intact protein yield Phosphatase Inhibitor Cocktail 2 inhibits tyrosine protein phosphatases acid phosphatases and alkaline phosphatases Phosphatase Inhibitor Cocktail 2 has been optimized and tested in cell extracts from multiple animal tissues This Phosphatase Inhibitor Cocktail 2 contains Sodium orthovanadate Sodium molybdate Sodium tartrate Imidazole and Sodium Fluoride It is supplied as a ready-to-use solution in ddH2O
Supply Solutions RNase A, 25 mg; from bovine pancreas (Roche); pkg of 100 mg (10109169001) pkg of 25 mg (10109142001)
RNase A, 25 mg; from bovine pancreas (Roche); pkg of 100 mg (10109169001) pkg of 25 mg (10109142001)
New England Biolabs, Inc. Endoproteinase GluC – 50 µg
Endoproteinase GluC is a serine proteinase which preferentially cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues at a rate 100-300 times slower than at glutamic acid residues.
New England Biolabs, Inc. NcoI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence C^CATG_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Sigma Aldrich Fine Chemicals Biosciences Protease from Streptomyces griseus, 9036-06-0, MFCD00132092, 1 g
Type XIV, ≥3.5 units/mg solid, powder, Synonym: Actinase E, Pronase E. Highly stable in pH range of 5.0 to 9.0, with peak activity at pH 8.8. Compatible with many DNA and RNA isolation buffers. Broad substrate specificity.
Sigma Aldrich Fine Chemicals Biosciences Lysozyme from chicken egg white, 12650-88-3, MFCD00131557, 5 g
Lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein, Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase. Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts. Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
LGC GENOMICS LLC (Lucigen) RNA 5' Polyphosphatase, 200U @ 20 U/LDephosphorylate tri- and di-phosphorylated RNA molecules while avoiding 5'-capped and mono-phosphorylated RNAs.
RNA 5 Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the and phosphates from 5-triphosphorylated RNA (such as primary RNA transcripts): 5 pppNOH 3 5 pNOH 3 + 2 Pi
Sigma Aldrich Fine Chemicals Biosciences Lysostaphin from Staphylococcus staphylolyticus lyophilized powder, Protein 50-70 % by biuret, >=500 units/mg protein | 9011-93-2 | MFCD00131556 | 1MG
Lysostaphin from Staphylococcus staphylolyticus lyophilized powder, Protein 50-70 % by biuret, >=500 units/mg protein | 9011-93-2 | MFCD00131556 | 1MG
Qiagen Beverly Inc T4 DNA LIGASE (RAPID)
DNA Ligase; T4 DNA Ligase (Rapid); Conc. 600,000 U/ml; 240,000U; incl. 10X buffer and 2X rapid ligation buffer
Takara Bio Recombinant DNase I (RNase-free), 1,000 Units
Recombinant DNase I (RNase-free) is an endonuclease that catalyzes, to the same degree, the random degradation of both single- and double-stranded DNA and produces 5'-P terminal oligonucleotides. This enzyme does not exhibit protease activity. This enzyme is stable around its optimum neutral pH range and is suitable for RNA preparation at neutral pH. 1000 Units
New England Biolabs, Inc. RNA 5’ Pyrophosphohydrolase (RppH) – 200 units
The bacterial RNA 5' Pyrophosphohydrolase (RppH) removes pyrophosphate from the 5' end of triphosphorylated RNA to leave a 5' monophosphate RNA. The RppH protein was also known as NudH/YgdP which can split Ap5A to ADP and ATP.